Viscose liquids are challenging to pipette because of their high viscosity and propensity to adhere to surfaces. On the other hand, with the appropriate method and choice of pipette tip, accurate pipetting of viscous liquids is possible. The following guidelines should be followed when pipetting viscous liquids:

  • Use wide-bore pipette tips: Because of their wider internal diameter, viscous liquids can flow more easily into and out of wide-bore pipette tips. In addition to improving accuracy, this can reduce the amount of liquid that remains in the tip after pipetting.

 

  • Pre-wet the pipette tip: By pre-wetting the pipette tip with a small amount of viscous liquid, you can reduce the amount of liquid that adheres to the inside of the tip. This can improve accuracy and lessen the chance of air bubbles forming in the tip.

 

  • Use a slow pipetting speed: Starting slowly is a crucial tip when pipetting viscous liquids. By doing this, air bubbles will be less likely to form and the liquid will have more time to enter and exit the tip.

 

  • Use reverse pipetting: Reverse pipetting is one way to improve viscous liquid pipetting accuracy. To reverse the pipette, first, fill its tip with the desired volume of liquid. Next, carefully refill the reservoir with a small amount of liquid. The vacuum that is created in the tip when you expel the liquid will assist in drawing out the remaining liquid.

 

  • Touch the pipette tip to the side of the vessel: After expelling the liquid, touch the pipette's tip to the side of the vessel to remove any remaining liquid. You can ensure that the proper amount of liquid is being dispensed by carrying out this action.

 

  • Change pipette tips frequently: When pipetting viscous liquids, it's important to change the pipette tips This is because viscous liquids have the potential to adhere to the tip's interior and impede the accuracy of pipetting.

 

  • Use a positive displacement pipette: It might be necessary to use a positive displacement pipette when pipetting extremely viscous liquids. Positive displacement pipettes use a plunger to dispense liquid, making them more accurate than air displacement pipettes for viscous liquids.

 

  • Calibrate your pipette: Regular calibration of your pipette is essential to ensure that the correct amount of liquid is being dispensed. This is especially important when pipetting viscous liquids.

 

Following these best practices will enable you to pipette viscous liquids with greater accuracy and precision.